Review



src 32g6 rabbit mab  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc src 32g6 rabbit mab
    Src 32g6 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/bio_rxiv__64898__2026__04__02__716058-64-15-78
    Average 86 stars, based on 1 article reviews
    src 32g6 rabbit mab - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Cell Migration Assay:

    Article Title: Podoplanin promotes cell migration via the EGF-Src-Cas pathway in oral squamous cell carcinoma cell lines.
    Article Snippet: .. Cell migration assay (scratch assay) Cell lines were seeded in 12-well tissue culture slides Antibodies Dilution Source β-actin 1:200 Santa Cruz Biotechnology, CA, USA GAPDH 1:500 Santa Cruz Biotechnology, CA, USA podoplanin 1:50 Santa Cruz Biotechnology, CA, USA p130Cas 1:100 Santa Cruz Biotechnology, CA, USA Src Rabbit mAb 1:1000 Cell Signaling Technology, Denvers, USA Non-phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA Phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA at 5 × 105 cells/well and incubated at 37°C for 24 h. One microliter of mixture (final concentration of siRNA for podoplanin, 10 nM) was applied to each well of a 12-well tissue culture slide and incubated for 24 h. A scratch through the central axis of the plate was gently made using a pipette tip, and the cells were then incubated with EGF (100 ng/mL). ..

    Wound Healing Assay:

    Article Title: Podoplanin promotes cell migration via the EGF-Src-Cas pathway in oral squamous cell carcinoma cell lines.
    Article Snippet: .. Cell migration assay (scratch assay) Cell lines were seeded in 12-well tissue culture slides Antibodies Dilution Source β-actin 1:200 Santa Cruz Biotechnology, CA, USA GAPDH 1:500 Santa Cruz Biotechnology, CA, USA podoplanin 1:50 Santa Cruz Biotechnology, CA, USA p130Cas 1:100 Santa Cruz Biotechnology, CA, USA Src Rabbit mAb 1:1000 Cell Signaling Technology, Denvers, USA Non-phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA Phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA at 5 × 105 cells/well and incubated at 37°C for 24 h. One microliter of mixture (final concentration of siRNA for podoplanin, 10 nM) was applied to each well of a 12-well tissue culture slide and incubated for 24 h. A scratch through the central axis of the plate was gently made using a pipette tip, and the cells were then incubated with EGF (100 ng/mL). ..

    Incubation:

    Article Title: Podoplanin promotes cell migration via the EGF-Src-Cas pathway in oral squamous cell carcinoma cell lines.
    Article Snippet: .. Cell migration assay (scratch assay) Cell lines were seeded in 12-well tissue culture slides Antibodies Dilution Source β-actin 1:200 Santa Cruz Biotechnology, CA, USA GAPDH 1:500 Santa Cruz Biotechnology, CA, USA podoplanin 1:50 Santa Cruz Biotechnology, CA, USA p130Cas 1:100 Santa Cruz Biotechnology, CA, USA Src Rabbit mAb 1:1000 Cell Signaling Technology, Denvers, USA Non-phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA Phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA at 5 × 105 cells/well and incubated at 37°C for 24 h. One microliter of mixture (final concentration of siRNA for podoplanin, 10 nM) was applied to each well of a 12-well tissue culture slide and incubated for 24 h. A scratch through the central axis of the plate was gently made using a pipette tip, and the cells were then incubated with EGF (100 ng/mL). ..

    Concentration Assay:

    Article Title: Podoplanin promotes cell migration via the EGF-Src-Cas pathway in oral squamous cell carcinoma cell lines.
    Article Snippet: .. Cell migration assay (scratch assay) Cell lines were seeded in 12-well tissue culture slides Antibodies Dilution Source β-actin 1:200 Santa Cruz Biotechnology, CA, USA GAPDH 1:500 Santa Cruz Biotechnology, CA, USA podoplanin 1:50 Santa Cruz Biotechnology, CA, USA p130Cas 1:100 Santa Cruz Biotechnology, CA, USA Src Rabbit mAb 1:1000 Cell Signaling Technology, Denvers, USA Non-phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA Phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA at 5 × 105 cells/well and incubated at 37°C for 24 h. One microliter of mixture (final concentration of siRNA for podoplanin, 10 nM) was applied to each well of a 12-well tissue culture slide and incubated for 24 h. A scratch through the central axis of the plate was gently made using a pipette tip, and the cells were then incubated with EGF (100 ng/mL). ..

    Transferring:

    Article Title: Podoplanin promotes cell migration via the EGF-Src-Cas pathway in oral squamous cell carcinoma cell lines.
    Article Snippet: .. Cell migration assay (scratch assay) Cell lines were seeded in 12-well tissue culture slides Antibodies Dilution Source β-actin 1:200 Santa Cruz Biotechnology, CA, USA GAPDH 1:500 Santa Cruz Biotechnology, CA, USA podoplanin 1:50 Santa Cruz Biotechnology, CA, USA p130Cas 1:100 Santa Cruz Biotechnology, CA, USA Src Rabbit mAb 1:1000 Cell Signaling Technology, Denvers, USA Non-phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA Phospho-Src (Ty527) Antibody 1:1000 Cell Signaling Technology, Denvers, USA at 5 × 105 cells/well and incubated at 37°C for 24 h. One microliter of mixture (final concentration of siRNA for podoplanin, 10 nM) was applied to each well of a 12-well tissue culture slide and incubated for 24 h. A scratch through the central axis of the plate was gently made using a pipette tip, and the cells were then incubated with EGF (100 ng/mL). ..

    Membrane:

    Article Title: Role of Src family kinases Fyn and Lyn in arenavirus infection.
    Article Snippet: .. The membrane was stained with the primary antibodies Phospho-Src Family Tyr416 (Cell Signaling Technology; dilution 1:1,000) or Src Rabbit mAb (Cell Signaling Technology; dilution 1:1,000). .. Densitometry analysis was performed using ImageJ over three independent experiments. siRNA-mediated gene knockdown Stock solutions (50 nM per well in 24-well plates) of siRNA listed in Table S1 (Gene Pharma) and siRNA control (named NC, UUC UCC GAA CGU GUC ACG UTT) were prepared using RNase-free water. siRNA transfection was performed with RNAiMAX (Invitrogen) according to instructions; the siRNA used in per well was at a final concen tration of 5 pmol.

    Staining:

    Article Title: Role of Src family kinases Fyn and Lyn in arenavirus infection.
    Article Snippet: .. The membrane was stained with the primary antibodies Phospho-Src Family Tyr416 (Cell Signaling Technology; dilution 1:1,000) or Src Rabbit mAb (Cell Signaling Technology; dilution 1:1,000). .. Densitometry analysis was performed using ImageJ over three independent experiments. siRNA-mediated gene knockdown Stock solutions (50 nM per well in 24-well plates) of siRNA listed in Table S1 (Gene Pharma) and siRNA control (named NC, UUC UCC GAA CGU GUC ACG UTT) were prepared using RNase-free water. siRNA transfection was performed with RNAiMAX (Invitrogen) according to instructions; the siRNA used in per well was at a final concen tration of 5 pmol.



    Similar Products

    src 32g6 rabbit mab  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc src 32g6 rabbit mab
    Src 32g6 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/bio_rxiv__64898__2026__04__02__716058-64-15-78
    Average 86 stars, based on 1 article reviews
    src 32g6 rabbit mab - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    phospho src family tyr416 d49g4 rabbit mab  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc phospho src family tyr416 d49g4 rabbit mab
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    Phospho Src Family Tyr416 D49g4 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/bio_rxiv__64898__2026__04__02__716058-74-6-39
    Average 86 stars, based on 1 article reviews
    phospho src family tyr416 d49g4 rabbit mab - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    p src  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc p src
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/Phospho-Src+(Ser17)+Rabbit+mAb/pm41923217-130-22-23
    Average 94 stars, based on 1 article reviews
    p src - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    anti src  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti src
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    Anti Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/Src+Rabbit+mAb/pmc12955691-78-47-48
    Average 96 stars, based on 1 article reviews
    anti src - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    phospho src y419 rabbit mab  (Huabio Inc)
    86
    Huabio Inc phospho src y419 rabbit mab
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    Phospho Src Y419 Rabbit Mab, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/anti+atm+mabhuabio+phospho+rabbit+recombinant+s1981/pmc13059770-339-0-40
    Average 86 stars, based on 1 article reviews
    phospho src y419 rabbit mab - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    src rabbit mab  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc src rabbit mab
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    Src Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/Src+Rabbit+mAb/pm41914886-205-17-20
    Average 96 stars, based on 1 article reviews
    src rabbit mab - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    rabbit monoclonal anti src  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc rabbit monoclonal anti src
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    Rabbit Monoclonal Anti Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/Src+Rabbit+mAb/pm41901318-133-67-74
    Average 96 stars, based on 1 article reviews
    rabbit monoclonal anti src - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti phospho src tyr416  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti phospho src tyr416
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    Anti Phospho Src Tyr416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/Phospho-Src+Family+(Tyr416)+Rabbit+mAb/pm41866450-70-43-49
    Average 96 stars, based on 1 article reviews
    anti phospho src tyr416 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    c src family phospho tyr416  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc c src family phospho tyr416
    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.
    C Src Family Phospho Tyr416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+rabbit+mab/Phospho-Src+Family+(Tyr416)+Rabbit+mAb/pm41871717-58-48-63
    Average 96 stars, based on 1 article reviews
    c src family phospho tyr416 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation (Tyr416), and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.

    Journal: bioRxiv

    Article Title: Heterogeneous Sensitivity to Src Inhibitors in Oral Squamous Cell Carcinoma and Its Implications for Combination Therapy with Cisplatin

    doi: 10.64898/2026.04.02.716058

    Figure Lengend Snippet: A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation (Tyr416), and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.

    Article Snippet: The following primary antibodies were used: Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb (#6943), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb (#4370), Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb (#4511), and Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (#4668) (all from Cell Signaling Technology).

    Techniques: Western Blot, Protein-Protein interactions, Control, Standard Deviation, Activity Assay, Phospho-proteomics